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New Tools to Perform Super Resolution Microscopy in Live Cells.

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New Tools for Fluorescent Protein Tagging to Perform Super Resolution Microscopy in Live Cells.

Twenty-five years ago, Green Fluorescent Protein (GFP) revolutionized cell biology. It allowed scientists, for the first time, to visualize proteins in live cells. Over the past decade an elegant approach for fluorescence labeling of proteins in live cells that is superior to GFP has been introduced. This approach relies on adding unnatural amino acids to proteins in cells.

Last month, the laboratories of Ben-Gurion University of the Negev’s Prof. Natalie Elia and Dr. Eyal Arbely published two papers in BMC Biology and Nanoscale offering new tools to cell biologists to help them embrace this labeling technique and demonstrating its applicability to super resolution microscopy and quantitative live cell imaging.

“By applying this labeling approach, we can get a glimpse into protein dynamics in real time. For a cell biologist, this is like discovering a new galaxy” says Prof. Elia.

It is too early to tell whether this labeling approach will change cell biology, but it clearly has the potential.

Prof. Elia is a member of the Department of Life Sciences and Dr. Arbely is a member of the Department of Chemistry, Faculty of Natural Sciences at BGU. Both are members of the National Institute for Biotechnology in the Negev.

The research was funded by a European Research Council (ERC) Starting Grant and the Israel Science Foundation.

microscopía de súper resolución

Twenty-five years ago, Green Fluorescent Protein (GFP) revolutionized cell biology. It allowed scientists, for the first time, to visualize proteins in live cells. Over the past decade an elegant approach for fluorescence labeling of proteins in live cells that is superior to GFP has been introduced. This approach relies on adding unnatural amino acids to proteins in cells.

Last month, the laboratories of Ben-Gurion University of the Negev’s Prof. Natalie Elia and Dr. Eyal Arbely published two papers in BMC Biology and Nanoscale offering new tools to cell biologists to help them embrace this labeling technique and demonstrating its applicability to super resolution microscopy and quantitative live cell imaging.

“By applying this labeling approach, we can get a glimpse into protein dynamics in real time. For a cell biologist, this is like discovering a new galaxy” says Prof. Elia.

It is too early to tell whether this labeling approach will change cell biology, but it clearly has the potential.

Prof. Elia is a member of the Department of Life Sciences and Dr. Arbely is a member of the Department of Chemistry, Faculty of Natural Sciences at BGU. Both are members of the National Institute for Biotechnology in the Negev.

The research was funded by a European Research Council (ERC) Starting Grant and the Israel Science Foundation.

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